A spatially specified systems pharmacology therapy for axonal recovery after injury

There are no known drugs or drug combinations that promote substantial central nervous system axonal regeneration after injury. We used systems pharmacology approaches to model pathways underlying growth and identify a four-drug combination regulates multiple subcellular processes in the cell body axons using optic nerve crush rats. intravitreally injected agonists HU-210 (cannabinoid receptor-1) IL-6 (interleukin 6 receptor) stimulate retinal ganglion cells for growth. applied, gel foam at site of injury, Taxol stabilize growing microtubules, activated protein C clear debris field since computational models predicted this regulating two cone produces synergistic Physiologically, treatment restored preserved pattern electroretinograms some animals had detectable visual evoked potentials brain behavioral optokinetic responses. Morphology experiments show protects promotes regrowth chiasm beyond. conclude spatially targeted is therapeutically relevant can restore limited functional recovery.